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MANDATORY DISCUSSION: Bacterial Transformation

MANDATORY DISCUSSION: Bacterial Transformation

Q Briefly describe steps of the transformation procedure. In your discussion, make sure that questions 1-6 are answered. 1. What is "transformation"? 2. What are competent cells? 3. What is the function of CaCl2 ? 4. What is heat shock? 5. What is the function of ampR gene"? 6- How do we select the transformed bacteria?

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1. 1. Transformation is profound, fundamental change, altering the very nature of something. Transformational change is both radical and sustainable.